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KMID : 1144820180240040398
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대한의생명과학회지
2018 Volume.24 No. 4 p.398 ~ p.404
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Effect of Ethanol Extracts from Defatted Perilla frutescens on LPS-induced Inflammation in Mouse BV2 Microglial Cells
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Lee Sung-Gyu
Kang Hyun
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Abstract
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To evaluate the antioxidant and anti-neuroinflammatory effects of defatted Perilla frutescens extract (DPE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Cell viabilities were estimated by MTT assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators such as nitric oxide (NO), inducible NO synthase (iNOS), Cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). Pretreatment with DPE prior to LPS treatment significantly inhibited excessive production of NO (10, 25, 50, 75, and 100 μg/mL) in a dose-dependent manner, and was associated with down regulation of expression of iNOS and COX-2. DPE also suppressed the LPS-induced increase in PGE2 level (10, 25, 50, 75, and 100 μg/mL) in BV-2 cells. Therefore, DPE can be considered as a useful therapeutic and preventive approach for the treatment of several neurodegenerative diseases.
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KEYWORD
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Defatted Perilla frutescens, Anti-inflammatory activity, Microglial cells, INOS, COX-2
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